Research articles
ScienceAsia 50 (2024):ID 2024041 1-8 |doi:
10.2306/scienceasia1513-1874.2024.041
Galangin modulation of the IL-23/IL-17 axis mitigates
ulcerative colitis through attenuation of oxidative/nitrative
stress and inflammation
Yang Zhanga, Ling Xiaob, Limin Yangc,*
ABSTRACT: The pathophysiology of Ulcerative Colitis (UC) involves the imbalance of pro-inflammatory cytokines,
including interleukin (IL)-23 and IL-17, which play a crucial role in the development and progression of UC.
Galangin (Gal), a natural flavonoid compound, has been shown to possess anti-inflammatory, antioxidant, and
immunomodulatory properties. Therefore, this study aimed to investigate the potential therapeutic effects of Gal
on a mouse model of dextran sulfate sodium (DSS)-induced UC by targeting the IL-23/IL-17 axis. Forty male
C57BL/6 mice were randomly divided into four groups and assessed clinical and histopathological features. The
colon tissues were collected for protein analysis using Western Blotting. Also, ELISA and colorimetric analysis were
used to measure cytokines and oxidative/nitrative stress markers, respectively. The expressions of iNOS and COX-2
were measured by real-time quantitative PCR (RT-qPCR). We found that Gal treatment significantly attenuated the
severity of UC, as evidenced by the improvement of clinical symptoms, histopathology, and reduced levels of proinflammatory cytokines, including IL-23 (fold change: 0.64; p < 0.05) and IL-17 (fold change: 0.56; p < 0.05).
Moreover, Gal treatment inhibited the activation of the NF-?B pathway. Furthermore, we demonstrated that Gal
treatment significantly suppressed oxidative/nitrative stress by reducing the expression levels of iNOS (fold change:
0.46; p < 0.01) and COX-2 (fold change: 0.52; p < 0.01), the two key enzymes involved in the production of
reactive oxygen/nitrogen species (ROS/RNS), and increasing the activity of antioxidant enzymes, including superoxide
dismutase and glutathione peroxidase. Our findings suggested that Gal targeting the IL-23/IL-17 axis improves UC by
suppressing oxidative/nitrative stress and inflammation.
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| a |
Pediatric Gastroenterology, Ganzhou Women and Children?s Health Care Hospital, Ganzhou 341000 China |
| b |
Anorectology, Binhai New Area Hospital of TCM, Tianjin 300450 China |
| c |
Anorectology, Xi?an Daxing Hospital, Xi?an 710082 China |
* Corresponding author, E-mail: yanglimin216415@sina.com
Received 18 Jul 2023, Accepted 23 Jan 2024
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