Research articles
ScienceAsia 52 (2026): 1-9 |doi:
10.2306/scienceasia1513-1874.2026.040
Exploration of bacteria isolated from black soldier fly larvae
for plant growth-promotion and organic acid production
Sirinapa Chungopasta, Supphawed Tussanakita, Chanwit Kaewtapeeb, Ratchanee Mingmac,*
ABSTRACT: Bacteria isolated from Black Soldier Fly (BSF) larvae have significant potential as effective sources of
beneficial microorganisms. The objective of this research was to investigate and classify the utility of bacteria isolated
from the BSF larvae. The isolation process involved examining the microorganisms? morphology and performing Gram
staining, followed by classification using the 16S rRNA gene. The activities of the isolates?including ammonium
release, phosphate solubilization, plant hormone production, and organic acid generation?were thoroughly analyzed.
Atotal of twelve bacterial isolates were obtained from the BSF larvae. Selected isolates with notable activity levels and
non-pathogenic characteristics were subjected to further investigation. Among these, isolate BSFL07 was identified
as Bacillus siamensis, which produced 25.12 ?g/ml of indole-3-acetic acid (IAA) and exhibited the highest production
of isovaleric acid (22.73 ?g/ml). Isolate BSFL08 was identified as Bacillus stercoris, producing 14.38 ?g/ml of IAA.
Isolate BSFL11 was classified as Bacillus wiedmannii, producing 18.96 ?g/ml of IAA, and the highest levels of acetic
acid (137.1 ?g/ml) and isobutyric acid (21.08 ?g/ml). All three isolates exhibited phosphate-solubilizing activity
and limited nitrogen-fixation ability, which are key traits associated with plant growth promotion. Future studies will
evaluate the effects of growth stage, cultivation time, and medium C/N ratio on metabolite production to optimize
their application as biofertilizers.
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| a |
Department of Soil Science, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University,
Kamphaeng Saen Campus, Nakhon Pathom 73140 Thailand |
| b |
Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok 10900 Thailand |
| c |
Department of Science and Bioinnovation, Faculty of Liberal Arts and Science, Kasetsart University,
Kamphaeng Saen Campus, Nakhon Pathom 73140 Thailand |
* Corresponding author, E-mail: ratchanee.mi@ku.th
Received 21 May 2025, Accepted 23 Apr 2026
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