Research articles
ScienceAsia 51 (2023): 1-11 |doi:
10.2306/scienceasia1513-1874.2023.061
Functional characterization of GNA11 in esophageal
squamous cell carcinoma: bioinformatics and cellular
functional analysis
Yihe Houa,b,c,?, Yifan Chenga,b,c,?, Huimin Wangb,c,d, Ziqing Xua, Qian Yange, Jicun Zhuf, Tiandong Lia,b,c,
Yuanlin Zoua,b,c, Hua Yea,b,c, Keyan Wangb,c,g, Jianxiang Shib,c,g, Peng Wanga,b,c,*
ABSTRACT: Guanine nucleotide-binding protein subunit alpha 11 (GNA11) has an established role in various types
of cancer, however, its involvement in esophageal squamous cell carcinoma (ESCC) remains uncharted. Data from
The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) project were utilized to analyze GNA11
expression across various cancers, its functional enrichment pathways in ESCC, and its correlation with immune
infiltration. Single-cell RNA sequencing was used to quantify GNA11 expression across different cell types. Real
time quantitative PCR and Western blotting were conducted to verify the GNA11 expression levels. ESCC cells
KYSE150 and TE1 were transduced to construct knockdown and overexpression models. Proliferation was assessed
using growth curve analysis, Cell Counting Kit-8 (CCK8) assays, and colony formation assays. Wound healing and
transwell assays evaluated the impact of GNA11 on cancer cell migration and invasion. Functional enrichment analysis
revealed associations with key pathways. Additionally, GNA11 interacts with proteins involved in G protein-coupled
receptor and chemokine signaling pathways. Single-cell RNA sequencing and immune infiltration analyses indicated
a positive correlation between GNA11 expression and tumor microenvironment components. In vitro experiments
showed that GNA11 overexpression enhanced the proliferation, migration, and invasion capabilities of ESCC cells,
whereas GNA11 knockdown produced the opposite effects. This study is the first to elucidate the critical role of GNA11
in the development and progression of ESCC. Abnormally high expression of GNA11 promotes ESCC and potentially
influences interactions between stromal cells and malignant cells. Thus, GNA11 could serve as a promising therapeutic
target for ESCC treatment.
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| a |
College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001 China |
| b |
Henan Key Laboratory of Tumor Epidemiology & Henan International Joint Laboratory of Tumor Biomarkers and
Molecular Imaging, Zhengzhou University, Zhengzhou, Henan 450052 China |
| c |
National Key Laboratory of Metabolism Disorder and Esophageal Cancer Prevention & Treatment, Zhengzhou
University, Zhengzhou, Henan 450052 China |
| d |
Henan General Hospital, Zhengzhou, Henan 450003 China |
| e |
Prenatal Diagnosis Center, The Third Affiliated Hospital of Zhengzhou University/Maternal and Child Health
Hospital of Henan Province, Zhengzhou, Henan 450052 China |
| f |
Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052 China |
| g |
Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan 450052 China |
* Corresponding author, E-mail: wangpeng1658@hotmail.com
Received 15 Aug 2024, Accepted 9 Jun 2025
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