Research articles
ScienceAsia 51S (2025):ID 2025s027 1-6 |doi:
10.2306/scienceasia1513-1874.2025.s027
Comparative analysis of bioactive compounds and
anti-inflammatory effects via COX-2 inhibitory activity in
forest-derived and tissue-cultured extracts of umbrella moss
(Rhodobryum giganteum (Schwägr.) Par.)
Hataichanok Panditha,b,*, Kullanun Mekawana, Pattarasuda Chayapakdeea,b, Witthawin Baiubola,
Nipitpawn Limpanicha, Rujipas Yongsawasa, Narin Printarakula,*
ABSTRACT: Rhodobryumgiganteum(umbrellamoss)isatraditionalChinesemedicinalplantprimarilyusedfortreating
heart diseases through anti-inflammatory mechanisms. Its key bioactive compounds are 7,8-dihydroxycoumarin and
p-hydroxycinnamic acid. Since umbrella moss grows in humid mountain regions, overharvesting wild populations
risks extinction and contamination with heavy metals. To address this, tissue culture techniques were developed
to propagate the moss sustainably. This study compares the bioactive compounds content and anti-inflammatory
efficacy via cyclooxygenase-2 (COX-2) inhibitory activity of forest-derived and tissue-cultured R. giganteum extracts.
Both samples were extracted using 60% ethanol and analyzed by high-performance liquid chromatography for active
compounds, followedbyenzyme-linkedimmunosorbentassayforCOX-2inhibition. Thetissue-cultured moss hadmuch
higher amounts of 7,8-dihydroxycoumarin and p-hydroxycinnamic acid than the natural forest moss. Specifically, it
contained about 15 times more 7,8-dihydroxycoumarin (6.16?1.25 ?g/ml vs. 0.41?0.03 ?g/ml) and nearly 5 times
more p-hydroxycinnamicacid(17.63?0.47?g/mlvs.3.69?0.06?g/ml). Theseresultsdemonstratethattissueculture
not only supports sustainable propagation but also enhances bioactive compound production and therapeutic efficacy.
This preliminary in vitro study provides a foundation for developing pharmaceutical products from R. giganteum while
conserving natural populations. Further cell-based assays and in vivo studies are necessary to evaluate the therapeutic
potential.
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| a |
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200 Thailand |
| b |
Research Center of Deep Technology in Beekeeping and Bee Products for Sustainable Development Goals (SMART
BEE SDGs), Chiang Mai University, Chiang Mai 50200 Thailand |
* Corresponding author, E-mail: hataichanok.p@cmu.ac.th, narin.printarakul@cmu.ac.th
Received 1 Dec 2024, Accepted 0 0000
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