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Research articles

ScienceAsia (): 392-399 |doi: 10.2306/scienceasia1513-1874...392


Anti-inflammatory activity of a Vernonia cinerea methanolic extract in vitro


Aurasorn Saraphanchotiwitthayaa,b,*, Pattana Sripalakitb,c

 
ABSTRACT:     Vernonia cinerea Less. (Asteraceae) is a herbaceous plant commonly found in SE Asia. Different parts of V. cinerea have been used in various folklore medicine applications, including reducing inflammation and detoxification. This study investigates the effects of V. cinerea methanolic extract on the mouse immune system in vitro. Cytokine detection of ConA-stimulated splenocytes using ELISA, mitogen-induced splenocyte proliferation, and phagocytic activity were assayed. The results show that the extract shifted the Th1/Th2 balance towards a Th2 type response. Additionally, Th1- and Th2-related cytokine production correlated with lymphocyte proliferation by selective activation of T-cells into Th2-cells and suppression of Th1-cell proliferation. Our results confirmed the traditional applications of V. cinerea on immune-related remedies and anti-inflammation, suggesting a potential therapeutic application of this plant in the treatment of diseases associated with soma carisoprodol cytokine production and inflammation.

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a Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, Thailand
b Pharmaceutical Biotechnology Research Unit, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, Thailand
c Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, Thailand

* Corresponding author, E-mail: aurasorns@nu.ac.th

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Received 6 Aug 2014, Accepted 2 Nov 2015