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Research articles

ScienceAsia 40 (2014): 121-124 |doi: 10.2306/scienceasia1513-1874.2014.40.121

The phytochemical properties of a new citrus hybrid (Citrus hystrix × Citrus microcarpa)

Abdul-Halim Yahya, Gun-Hean Chong*, Chin-Ping Tan

ABSTRACT:     The Merdeka lime is a new citrus hybrid with strong resistance against pests and disease. This work aims to provide information about the total phenolic content (TPC), total flavonoid (TF) content and antioxidant potential of the leaves, peel and pulp of this new hybrid along with those of its parent plants (Citrus hystrix and C. microcarpa). The TPC and TF contents were determined based on a colorimetric method, while antioxidant levels were determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the ferric-reducing antioxidant power (FRAP Nike shoes) techniques. The results revealed that Merdeka lime contained the highest TPC (4600±140 µg gallic acid equivalents per gram of dry weight) and DPPH value (4260±30 µg Trolox equivalents per gram of dry weight) in the leaves and the highest TF (16 400±300 µg quercetin equivalents per gram dry weight) and FRAP value (13 430±60 µg ascorbic acid equivalent per gram of dry weight) in the peel. The antioxidant activities of Merdeka lime were similar to the parent plants.

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Department of Food Technology, Faculty of Food Science and Technology, Putra Malaysia University, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

* Corresponding author, E-mail: gunhean@upm.edu.my

Received 10 Apr 2013, Accepted 19 Jan 2014