| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 51 Number 1
Volume 50S Number 1
Volume 50 Number 6
Volume 50 Number 5
Volume 50 Number 4
Volume 50 Number 3
Earlier issues
Volume  Number 

previous article next article

Research articles

ScienceAsia (): 26-35 |doi: 10.2306/scienceasia1513-1874...026


Characterization of xylose-utilizing yeasts isolated from herbivore faeces in Thailand


Wanlapa Lorliama, Ancharida Akaracharanyab, Sasitorn Jindamorakotc, Surisa Suwannarangseed, Somboon Tanasupawata,*

 
ABSTRACT:     A total of 39 xylose-utilizing yeast strains were isolated from herbivore faeces in Thailand. They were identified as Candida tropicalis (32 isolates), Candida albicans (1 isolate), Pichia terricola (1 isolate), Trichosporon mycotoxinivorans (2 isolates), Sporopachydermia lactativora (2 isolates) and Zygoascus meyerae (1 isolate) based on their morphological, cultural, physiological and biochemical characteristics including the sequence analysis of the D1/D2 region of the large-subunit ribosomal DNA. Thirty seven isolates could ferment xylose to ethanol. Zygoascus meyerae E23 isolated from elephant faeces produced the highest ethanol concentration (3.61 g/l after 72 h). C. tropicalis A26 isolated from cow faeces produced the highest xylitol concentration (43.79 g/l) which corresponded to 0.71 g xylitol/g xylose after 24 h. C. tropicalis A26 xylose reductase showed 98.4% identity and 99.0% similarity to C. tropicalis (ABX60132C) xylose reductase, and showed the tetra-amino acid motif (Ile-Pro-Lys-Ser) which is conserved among NADPH-dependent xylose reductase.

Download PDF

26 Downloads 1700 Views

a Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330 Thailand
b Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330 Thailand
c BIOTEC Culture Collection, Bioresources Technology Unit, National Centre for Genetic Engineering and Biotechnology (BIOTEC), Pathum Thani 12120 Thailand
d Enzyme Technology Laboratory, Bioresources Technology Unit, National Centre for Genetic Engineering and Biotechnology (BIOTEC), Thailand Science Park, Pathum Thani 12120 Thailand

* Corresponding author, E-mail: Somboon.T@chula.ac.th

Received 18 May 2012, Accepted 26 Nov 2012