| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 50 Number 1
Volume 49 Number 6
Volume 49 Number 5
Volume 49S Number 1
Volume 49 Number 4
Volume 49 Number 3
Earlier issues
Volume  Number 

next article

Research articles

ScienceAsia 36 (2010): 259-270 |doi: 10.2306/scienceasia1513-1874.2010.36.259

Changes in the 2DE protein profiles of chilli pepper (Capsicum annuum) leaves in response to Fusarium oxysporum infection

Aphinya Wongpiaa, Khemika Lomthaisongb,*

ABSTRACT:     Wilt disease caused by Fusarium oxysporum f. sp. capsici is a major problem of chilli pepper production worldwide that calls for a better understanding of defensive mechanisms in the chilli plant. We used a proteomic technique to investigate protein responses of chilli pepper to F. oxysporum f. sp. capsici. Two cultivars of resistant (Mae Ping 80) and susceptible (Long Chilli 455) plants were cultured in vitro. Chilli plants at 6-week growth were then infected with a suspension of F. oxysporum f. sp. capsici or distilled water used as a control. After 48 h of infection, proteins were extracted and analysed using 2DE to identify the responsive proteins. At least 9 spots were differentially expressed in the resistant cultivar (5 increasing, 4 decreasing) and 1 supplementary; while 15 increasing, 11 decreasing, and 11 supplementary protein spots were found in the susceptible cultivar. These proteins were then identified by MALDI-TOF MS combined with bioinformatics methods. Some of the induced proteins e.g., NADPH HC toxin reductase, serine/threonine protein kinase, and 1-aminocyclopropane-1-carboxylate synthase 3 are involved in plant defence mechanism. In order to determine the Fusarium wilt protective proteins in chilli plant, the protein patterns of healthy resistance were compared with those of susceptible cultivars. Interestingly, resistance showed higher expression of proteins related to ROS detoxification. Moreover, the ability of chilli plant to resist Fusarium wilt disease was related to the expression of non-inducible immunity 1 protein.

Download PDF

31 Downloads 1270 Views

a Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
b Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand

* Corresponding author, E-mail: khemlo@kku.ac.th

Received 1 Dec 2009, Accepted 21 Sep 2010