Research articles
ScienceAsia 50 (2024):ID 2024074 1-8 |doi:
10.2306/scienceasia1513-1874.2024.074
Study of mating type ratio and colony sectorization of asexual
spores on the progress of Cordyceps militaris degeneration
Yu-jie Fenga,?, Yun Zhub,?, Yong-mei Lic, Yan-fei Sund, Jian-bo Zhud,*
ABSTRACT: Cordyceps militaris stands out as one of the limited species within the Cordyceps genus fungal that has the
capability of being cultivated artificially, but the fruiting bodies yield was reduced along with subculture which have
seriously hindered the development of the C. militaris industry. This paper primarily studied effect of asexual spores on
fruiting bodies production of C. militaris. First, the mating type (MAT) and distribution ratio of individual asexual spores
were investigated. Further, single asexual spores were randomly selected to subculture individually until visible colony
sectorization produced; then, the fruiting bodies production and the genetic diversity by sequence-related amplified
polymorphism (SRAP) polymerase chain reaction (PCR) of asexual spores from different colony morphologies were
investigated. The results showed that C. militaris single asexual spores were homokaryotic. Asexual spores ratio of
MAT-1-1:MAT-1-2 was about 1:2 in normal strain, which can produce fruiting bodies; but the mating type of single
asexual spores from degenerated strains were the same. The strain of CMS1-3 (MAT-1-2) produced sectors after subculturing for 3 generations; asexual spores of sectorial colony could not produce fruiting bodies after cross-mating. The
SRAP PCR results showed that the DNA of asexual spores isolated from different colonies have changed during subculturing. The results indicated that the imbalance of MAT ratio in individual asexual spores, along with the creation
of mutants that fail to produce fruiting bodies, can significantly reduce the production of C. militaris fruiting bodies,
potentially leading to a total loss of their ability to form fruiting bodies.
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| a |
Department of Biological Science and Technology, Jinzhong University, Shanxi 030619 China |
| b |
School of Pharmacy, Shihezi University, Xinjiang 832003 China |
| c |
National Key Laboratory for Biological Breeding of Tropical Crops, Hainan University, Hainan 570228 China |
| d |
School of Life Science, Shihezi University, Xinjiang 832003 China |
* Corresponding author, E-mail: zjbshz@126.com
Received 26 Nov 2023, Accepted 27 May 2024
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