Research articles
ScienceAsia 50 (2024):ID 2024069 1-9 |doi:
10.2306/scienceasia1513-1874.2024.069
Alteration of antimicrobial phenotypic resistance of E. coli in
municipal wastewater treatment process and receiving canal
water in Bangkok, Thailand
Wilai Chiemchaisria,*, Chart Chiemchaisria, Nopparat Patchaneeb, Toru Watanabec
ABSTRACT: The phenotypic prevalence of antibiotic-resistant E. coli (ARE) in the sewage of Bangkok City, Thailand,
during the conventional activated sludge (CAS) process was evaluated. The relationship of ARE in the CAS
effluent/receiving water was investigated. The CAS E. coli isolates were tested against 20/21 antibiotics in 6 classes:
4 aminoglycosides, A; 3 tetracyclines, T; 1 chloramphenicol, C; 11 quinolones, Q; 1 sulfonamide, S; and 1 betalactam, B. The results showed that the sewage contained 84% ARE, of which 28% was single drug-resistance and 56%
was multidrug-resistant E. coli (MRE). Poor removals of ARE/MRE were found in the CAS. Inversely, a more susceptive
E. coli population to all tested antibiotics was detected in the CAS effluent (24%). When MRE was classified into low
(L)-, medium (M)-, and high (H)-levels based on number of resistant antibiotics, it showed that the effluent L-MRE
(2 to 5 antibiotics) was highest (46%) compared to the others (29?38%), while H-MRE (11 to 16 antibiotics) was
relatively dominant in the dewatered sludge (24%). Additionally, Q-S-T was the most phenotypic resistant pattern in
sludge, while Q-S was highly frequent in effluent. ARE in receiving water showed a high correlation to MRE of CAS
effluent along receiving water due to high contamination of coliforms and E. coli.
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a |
Department of Environmental Engineering, Faculty of Engineering, Kasetsart University, Bangkok 10900 Thailand |
b |
Fisheries Program, Faculty of Agriculture Technology, Sakon Nakhon Rajabhat University, Sakon Nakhon 47000
Thailand |
c |
Department of Food, Life and Environmental Science, Faculty of Agriculture, Yamagata University, Yamagata
990-0021 Japan |
* Corresponding author, E-mail: fengwlc@ku.ac.th
Received 12 Sep 2023, Accepted 9 May 2024
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