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Saccharibacillus mori sp. nov., a novel indole-3-acetic acid-producing and phosphate-solubilizing bacterium isolated from a medicinal plant rhizosphere soil

Xue-Fei Han^a,b, Yang Deng^a,b, Li-Yan Yu^a, Hua-Hong Chen^c,*, Yu-Qin Zhang^a,b,*

 
ABSTRACT:     A Gram-positive, rod-like, and aerobic bacterium labeled JD154T was recovered from the rhizosphere soil of a mulberry tree, a medicinal plant, in Yunnan Province, China. It proved a closely knit relationship to the genus Saccharibacillus via the 16S rRNA gene sequence comparison, showing the highest similarity of 96.4% to Saccharibacillus kuerlensis DSM 22868T and Saccharibacillus sacchari DSM 19268T . The considerate low average nucleotide identity (77.1?80.0%) and digital DNA-DNA hybridization values (17.7?25.6%) with other Saccharibacillus strain genomes indicated strain JD154T as a new genetic species. It grew effectively between 10?37 ?C and a pH range of 5.0?9.0, optimally thriving at 20?30 ?C and a pH of 7.0?8.0. Major cellular fatty acids found in the strain were anteiso-C15:0, C16:0, iso-C14:0, and iso-C15:0. The primary respiratory quinone documented was menaquinone-7, and the polar lipids were diphosphatidylglycerol and phosphatidylglycerol. Evaluation of its genome revealed gene clusters potentially involved in processes such as phosphate solubilization and indole acetic acid biosynthesis. Evidence of these plant growthpromoting characteristics was confirmed by indole-3-acetic acid (IAA) production (11.04?0.04 ?g/ml) and phosphatesolubilizing (P-solubilizing) activity. Taking into account the unique genetic and phenotypic characteristics, strain JD154T is suggested to represent a novel species within the genus Saccharibacillus, proposed to be titled Saccharibacillus mori sp. nov.

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* Corresponding author, E-mail: huahongc@gmail.com, zhyuqin@126.com

Received 1 Aug 2023, Accepted 13 Jan 2024