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Research articles

ScienceAsia 49 (2023):ID 29-37 |doi: 10.2306/scienceasia1513-1874.2023.129


Probiotic characterization and in vitro functional properties of lactic acid bacteria isolated in Thailand


Jaruwan Sitdhipola,*, Kanidta Niwasabutraa, Neungnut Chaiyawana, Siritorn Teeraweta, Punnathorn Thaveethaptaikula, Sukanya Phuengjayaemb, Malai Taweechotipatrc,d, Somboon Tanasupawate, Pongsathon Phapugrangkula

 
ABSTRACT:     Fourteen lactic acid bacteria isolated from fermented foods and healthy animal feces in Thailand were characterized for their potential as probiotics. The 16S rRNA gene sequence analyses identified them as Lactiplantibacillus, Levilactobacillus, Limosilactobacillus, Lacticaseibacillus, Campanilactobacillus, Pediococcus, and Enterococcus genera. All strains survived in simulated gastrointestinal fluid (pH 2) and bile salt solution (pH 8) at over 70% and 63%, compared with initial cell concentration, respectively. In vitro adhesion testing showed their adhesive property of over 70%, while the results of antibiotic susceptibility indicated that all strains were susceptible to amoxicillin, ampicillin, erythromycin, chloramphenicol, clindamycin, imipenem, kanamycin, norfloxacin, penicillin, tetracycline, and vancomycin. All strains exhibited antimicrobial ability against Staphylococcus aureus TISTR 1466, Listeria monocytogenes TISTR 2196, Escherichia coli TISTR 780, Salmonella Enteritidis TISTR 2202, and Salmonella Typhimurium TISTR 292. Moreover, Limosilactobacillus reuteri MF67.1 and Companilactobacillus farciminis R7-1 showed bile salt hydrolase activity. Cellfree culture supernatants of all 14 strains were screened for immunomodulating effects on Tumor Necrosis Factor alpha (TNF-?) production. Lactiplantibacillus paraplantarum R26-3 and Lacticaseibacillus zeae M2/5 showed high inhibition of TNF-? production at 34% and 29% reduction, respectively; while the other 12 strains decreased TNF-? production at various lower levels. Results suggested that all 14 strains met the general criteria of probiotics. Lac. zeae M2/5, Lac. paraplantarum R26-3, Lim. reuteri MF67.1, and Com. farciminis R7-1 were interesting candidates for further studies as anti-inflammatory (M2/5, R26-3) or cholesterol-reducing agents (MF67.1, R7-1) in in vivo animal models.

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a Biodiversity Research Centre, Research and Development Group for Bio-Industries, Thailand Institute of Scientific and Technological Research, Pathum Thani 12120 Thailand
b Department of Microbiology, Faculty of Science, King Mongkut?s University of Technology Thonburi, Bangkok 10140 Thailand
c Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110 Thailand
d Center of Excellence in Probiotics, Strategic Wisdom and Research Institute, Srinakharinwirot University, Bangkok 10110 Thailand
e Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330 Thailand

* Corresponding author, E-mail: jaruwan_s@tistr.or.th

Received 10 Feb 2022, Accepted 18 Jul 2022