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Research articles

ScienceAsia 49 (2023):ID 85-93 |doi: 10.2306/scienceasia1513-1874.2023.141

Identification, in silico characterization, and expression analysis of NBS-LRR class of R genes against stem and crown rot disease in Trifolium alexandrinum L.

Bukhtawer Nasira, Siddra Ijaza,*, Imran Ul Haqb, Nasir Ahmad Khanb, Amer Habibb

ABSTRACT:     R genes with colossal contributions to disease resistance possess conserved domains with confounded roles. Based on the conserved domains, R genes encoded proteins are classified. The members of nucleotide-binding site, NBS-Leucine rich repeats, LRR class of R genes are significant candidates involved in plant disease-resistance pathways. In this study, the genetic elements contributing to stem and crown rot disease resistance were explored through transcriptome probing based on conserved NBS domain under disease stress in berseem clover. Expression analysis of identified gene sequences revealed their upregulation during stem and crown rot disease. The in silico characterization imparted strong support towards the predictive role of identified RGAs in disease resistance.

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a Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad 38000 Pakistan
b Department of Plant Pathology, University of Agriculture, Faisalabad 38000 Pakistan

* Corresponding author, E-mail:

Received 17 Dec 2021, Accepted 16 Aug 2022