| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 50 Number 2
Volume 50 Number 1
Volume 49 Number 6
Volume 49 Number 5
Volume 49S Number 1
Volume 49 Number 4
Earlier issues
Volume  Number 

previous article next article

Research articles

ScienceAsia 48 (2022):ID 635-641 |doi: 10.2306/scienceasia1513-1874.2022.087

Characterization, genome annotation, and antibacterial properties of Actinopolyspora saharensis BKK2

Sirilak Namwonga,*, Suchanat Pandeya, Masahiro Yukib, Takuji Kudob, Moriya Ohkumab, Somboon Tanasupawatc

ABSTRACT:     Strain BKK2 was highly similar to Actinopolyspora saharensis (A. saharensis) DSM 45459T based on phenotypic characteristics, chemotaxonomic properties, and genotypic features. It had NaCl ranges of 10?30% (w/v), light-yellow diffusion pigment, Type IV cell wall, Type A whole-cell sugar pattern, Type PIII phospholipid pattern, and no mycolic acid. Moreover, the genotypic features of the strain BKK2 were 98.56% average nucleotide identityBlast (ANIb), 99.03% average nucleotide identity-MUMmer (ANIm), and 90.90% digital DNA-DNA hybridization (dDDH). It was, therefore, identified as A. saharensis. Gene clusters of biosynthetic pathways of this strain are predicted to synthesize desferrioxamine E by complete biosynthesis genes (IucA/IucC and DesABC). A. saharensis BKK2 displayed antibacterial activity against E. coli ATCC 25922 and S. aureus ATCC 25923 at 1.5?2.0 folds weaker than the reference drugs. The results suggested that A. saharensis BKK2 produced at least one bioactive compound likely to be therapeutically useful in the future.

Download PDF

41 Downloads 1117 Views

a Department of Biotechnology, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok 10300 Thailand
b Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074 Japan
c Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330 Thailand

* Corresponding author, E-mail: sirilak.na@ssru.ac.th

Received 19 Nov 2021, Accepted 3 Apr 2022