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HPLC methods for quality control of Moringaÿoleifera extract using isothiocyanates and astragalin as bioactive markers

JinutdaÿEngsuwan^a,b, NetiÿWaranuch^b, NanteetipÿLimpeanchob^c, KornkanokÿIngkaninan^a,b,*

 
ABSTRACT:     Herbal extracts are gaining widespread acceptance as ingredients in cosmetic and health products. Moringa oleifera Lam. is an interesting candidate as it has multiple biological properties. However, methods for quality control of the use of M.ÿoleifera are still required. This study proposes the use of isothiocyanate compounds and astragalin as chemical markers to standardize M.ÿoleifera extracts. Both of these compounds are known to have anti-inflammation properties, and astragalin is also reported to have antioxidant properties. The HPLC-based cyclocondensation method was further developed and validated specifically for quantitative analysis of the isothiocyanate compounds in the M.ÿoleifera extracts, and the concentration of astragalin in the M.ÿoleifera extracts was determined by the HPLC method. Both methods gave acceptable linearity, accuracy, and precision with the limit of detection of 0.13ÿµg/ml for total isothiocyanate and 0.10ÿµg/ml for astragalin. The uses of the methods were demonstrated in the analyses of the components in the extracts taken variously from the leaves, immature seeds, mature seeds, petioles, and pods of the M.ÿoleifera tree. Our results suggest that the methods developed could be used in the quality control of M.ÿoleifera extracts to develop cosmetic and natural health products.

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* Corresponding author, E-mail: k_ingkaninan@yahoo.com

Received 18 Jan 2017, Accepted 12 Jul 2017