 BackShort Report ScienceAsia 15 (1989): 121-132 |doi: 10.2306/scienceasia1513-1874.1989.15.121
MODIFIED DYE BINDING METHOD FOR MEASURING TOTAL PROTEIN CONCENTRATION OF CEREBROSPINAL FLUID
VARUNEE KIETDURIYAKUL, SOONTHAREE TANTRARONGROJ, SURAWIT HERABUT AND PRASIT JEAMSANDUSADEEABSTRACT: The present method measures the absorbancy of supernatant after precipitation of protein in cerebrospinal fluid by TCA-Ponceau S dye. The optimum concentration of Ponceau S dye, amount of specimen and method of analysis which give good linearity, sensitivity, accuracy and precision have been determined. This modified method uses 2.94% trichloroacetic acid as in the Pesce-Strande method, 0.006% Ponceau S dye and 50 Department of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand. Received 26 August 1988
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l of specimen. The measured absorbancy of supernatant shows linearity up to 250 mg/dl of
protein. Optimum condition variance (OCV) are 3.22, 4.65 for high and normal values, while routine condition variance are 5.58, 8.46 for high and normal values. This modified method gives good correlation with the Pesce-Strande dye-binding method. The present method is appropriate for routine use in the laboratory because it is simple and has good accuracy and precision, wide range linearity, and needs only a small amount of sample. It is also suitable for small laboratories because it requires only simple equipments.
