ScienceAsia 9 (1983): 089-106 |doi: 10.2306/scienceasia1513-1874.1983.09.089

UPTAKE AND RELEASE OF NEUROTRANSMITTER-RELATED COMPOUNDS BY TWO POPULATIONS OF SYNAPTOSOMES ISOLATED FROM RABBIT RETINA

THYON CHENTANEZ^a and DIANNA A. REDBURN^b

ABSTRACT: Synapses of retina have high affinity uptake mechanisms which may play an important role in the chemical transmission processes of the retina. In this study, a comparison was made among the uptake systems of retinal synaptosomes obtained from the outer plexiform layer (OPL), the inner plexiform layer (IPL), and a retina homogenate (Hg).

Na+ and temperature sensitive uptake of choline (Ch), aspartate (Asp), gamma-aminobutyric acid (GABA), and dopamine (DA) was demonstrated in OPL and IPL synaptosomes of rabbit retina. The uptake rate of all four compounds was lower in OPL synaptosomes compared to IPL synaptosomes : 3 H-Ch 66%,,3 H-GABA 48%, 3 H-Asp 25% and H-DA 66.7 %.

Download PDF

a. Department of Physiology, Faculty of Science, Mahidol University Rama VI Rd., Bangkok, 10400 Thailand.
b. Department of Neurobiology and Anatomy, The University of Texas at Houston, Houston, Texas 77025, U.S.A.

Received 3 December 1982