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Research Article

ScienceAsia 2 (1976): 056-066 |doi: 10.2306/scienceasia1513-1874.1976.02.056

 

THE PRESENCE OF TWO IgG SUBCLASSES IN WATER-BUFFALO IMMUNOGLOBULINS

 

M.R. JISNUSON SVASTI, PRANIT PRAWATMUANG, CHAROENSRI VAJANAMARHUTUE, ANOCHA KAJADPHAI, SIRISAG WANGTHAMMANG and NAPASRI TALUPPHET

Summary: The immunoglobulins of a water-buffalo have been fractionated by ammonium sulphate precipitation and DEAE-cellulose chromatography into eight pools. These pools were analysed by polyacrylamide gel electrophoresis, analytical isoelectric focusing, hexose determination, and chemical typing. The results suggested that water-buffalo IgG consists of two subclasses, fast (IgG1) and slow (1gG2).

          In addition, the light chains were shown to be entirely of the lambda type. The C-terminal tryptic peptide. was purified and shown to contain an extra proline when compared to the equilivalent peptide from bovine light chains.

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Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand

Received 30 April 1976