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Research articles

ScienceAsia 44 (2018): 227-233 |doi: 10.2306/scienceasia1513-1874.2018.44.227

Utilization of cellulose microfiber from pineapple leaf as lipase immobilization support for highly retained activity, ease of separation and reusability

Thitinun˙Pradubsanga, Pranee˙Inprakhonb, Nisa˙Patikarnmonthonb, Taweechai˙Amornsakchaic,d,*

ABSTRACT:     We investigate the use of cellulose microfibre from pineapple leaf, referred to as pineapple leaf microfibre (PALMF), as a support for the immobilization of lipase AY from Candida rugosa. PALMF was produced from pineapple leaf waste with a very fine diameter of about 3 ?m. It disperses very well in aqueous media and can easily be filtered out. PALMF in the forms of loose fibre and of mat were used. Different amounts of enzyme were immobilized on these PALMFs and tested for their hydrolytic activity. The reusability of the immobilized enzyme was also studied. The results showed that, for the selected amount of lipase used, enzyme loading and PALMF form affect the hydrolytic activity of the enzyme. Immobilized enzymes display slightly lower hydrolytic activity than the free enzyme. The activity decreases when the enzyme loading (on PALMF) is increased. Lipase AY immobilized with optimum quantity (1.37 mg/g of PALMF support) displayed a 12% drop in activity from that of free enzyme. The crowding of enzyme molecules on the PALMF surface caused steric hindrance and reduced accessibility to the active site of the enzyme. So with the suitable amount of immobilized enzyme on PALMF, reasonable hydrolytic activity together with easy separation and reusability can be obtained. This method could aid significantly the use of enzyme in industrial processes.

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a Materials Science and Engineering Program, Faculty of Science, Mahidol University, Bangkok 10400 Thailand
b Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400 Thailand
c Polymer Science and Technology Program, Department of Chemistry and Centre of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Salaya, Nakhon Pathom 73170 Thailand
d Centre of Sustainable Energy and Green Materials, Faculty of Science, Mahidol University, Salaya, Nakhon Pathom 73170 Thailand

* Corresponding author, E-mail: taweechai.amo@mahidol.ac.th

Received 15 Oct 2017, Accepted 18 Aug 2018