Molecular cloning and identification of the hatching enzyme gene in Rondotia menciana

Shunming Tang^a,b,*, Huanying Wang^a,b,c, Xingkai Wang^a,b, Longshan Liu^a,b, Yuexia Pu^a,b,d, Xuezheng Wang^a,b, Qiaoling Zhao^a,b, Jialing Cheng^a,b, Xingjia Shen^a,b

ABSTRACT: Rondotia menciana Moore (Lepidoptera: Bombycidae) is a major pest of mulberry. We cloned the fulllength cDNA of the hatching enzyme from R. menciana eggs (RmHE) using the RACE-PCR and SMART cDNA synthesis technique. The cDNA is 1325 bp long and contains an ORF of 885 bp. It encodes 294 amino acid residues, including a putative signal peptide of 16 amino acid residues. Two conserved signature sequences of insect hatching enzyme (HE) harbour in the RmHE. The deduced amino acid sequence of RmHE has 30?84% identity to HE sequences in other insect species, and RmHE is most similar to that of wild silkworm HE. RmHE transcripts could be detected in R. menciana eggs at different developmental stages. Levels remained low during the early stages, increased greatly in 7-d eggs, and reached a maximum in 9-d eggs. Changes in the levels of RmHE transcripts occurred in accordance with the process of embryo development and eclosion. This indicated that RmHE plays an important role in these processes. RmHE transcripts were also detected in larval midguts, suggesting that RmHE is involved in food digestion. As far as we know, this is the first report on an HE gene in Lepidopteran pests and will offer some basic information for developing an efficient control method during the hatching process for agricultural Lepidopteran pests.

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^a	Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212003 China
^b	Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018 China
^c	Zhejiang Tianke Technology Development CO., LTD, Hangzhou 310012 China
^d	Guangxi General Station for Sericulture Technology Popularization, Nanning Guangxi 530007 China

* Corresponding author, E-mail: tangshunming2002@163.com

Received 1 Feb 2018, Accepted 28 Aug 2018

Molecular cloning and identification of the hatching enzyme gene in Rondotia menciana

Shunming Tanga,b,*, Huanying Wanga,b,c, Xingkai Wanga,b, Longshan Liua,b, Yuexia Pua,b,d, Xuezheng Wanga,b, Qiaoling Zhaoa,b, Jialing Chenga,b, Xingjia Shena,b

Shunming Tang^a,b,*, Huanying Wang^a,b,c, Xingkai Wang^a,b, Longshan Liu^a,b, Yuexia Pu^a,b,d, Xuezheng Wang^a,b, Qiaoling Zhao^a,b, Jialing Cheng^a,b, Xingjia Shen^a,b