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ScienceAsia 39(2013): 449-455 |doi: 10.2306/scienceasia1513-1874.2013.39.449


Efficient adventitious shoot regeneration from shoot tip culture of Vanda coerulea, a Thai orchid


Nipawan Jitsopakula, Kanchit Thammasirib,*, Keiko Ishikawac

 
ABSTRACT:     Vanda coerulea is a popular albeit endangered blue orchid of Thailand, which would be desirable to propagate using regeneration methods. Here, we studied the effects of culture media (Vacin and Went, and Murashige and Skoog), sucrose concentrations (0–30 g/l), and plant growth regulators (BA, TDZ, and NAA) on adventitious shoot regeneration from shoot tip culture of V. coerulea. Shoot tips cultured on modified Vacin and Went (VW) medium supplemented with 10 g/l sucrose showed higher shoot and root formations, as well as plantlet height than those cultured on Murashige and Skoog medium. Addition of 1 mg/l BA to the modified VW medium induced the best shoots and roots after 3 months of culture. The combination of 0.5 mg/l NAA and 2 mg/l TDZ gave the optimal number of roots per explant and plantlet height after 3 months of culture. Survival rate of plantlets cultured in the greenhouse was 100% after 3 months of culture. There were no differences in morphology or patterns of ploidy level between stock plants and regenerated plants.

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a Department of Agro-Industry, Faculty of Agriculture and Technology, Rajamangala University of Technology Isan, Surin Campus, Surin 32000 Thailand
b Department of Plant Science, Faculty of Science, Mahidol University, Bangkok 10400 Thailand
c Department of Research and Development, Japan Horticultural Production and Research Institute, Chiba 270-2221, Japan

* Corresponding author, E-mail: kanchitthammasiri@gmail.com

Received 13 Dec 2012, Accepted 20 May 2013