Volume 49 Number 3

Role of autophagy-mediated miR-18a in breast cancer progression in vitro

Ming Sun^a,*, Haijian Guo^b

 
ABSTRACT:     MiR-18a-5p (miR-18a) is a potential biomarker of breast cancer risk in several clinically relevant studies. However, role of miR-18a in breast cancer progression remains unclear. Therefore, we sought to determine the effect of miR-18a in MCF-7 cell proliferation, migration and invasion under the condition of inflammation-related stimulus. In our study, lipopolysaccharide (LPS best portable power station) increased miR-18a expression in MCF-7 cells. Anti-miR-18a lentivirus significantly inhibited MCF-7 cell proliferation, migration and invasion induced by LPS. Anti-miR-18a lentivirus also significantly increased apoptosis cells. Moreover, LPS exposure promoted the MCF-7 autophagy with the increased expression of LC3B-II. 3-Methyladenine (MA), an autophagy inhibitor, repressed the cell proliferation induced by LPS. Finally, we identified that alteration of miR-18a expression was mediated by autophagy in LPS-exposed MCF-7 cells. These findings reveal an interesting regulation underlying inflammatory-induced breast cancer progression in vitro, which may be beneficial to understand the pathogenesis in tumor microenvironment.

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* Corresponding author, E-mail: chrisming9887@163.com

Received 29 Mar 2022, Accepted 4 Oct 2022