| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 48 Number 6
Volume 48 Number 5
Volume 48 Number 4
Volume 48 Number 3
Volume 48 Number 2
Volume 48S Number 1
Earlier issues
Volume 46 Number 2 Volume 46 Number 3 Volume 46 Number 4

previous article next article

Research articles

ScienceAsia 46 (2020): 308-314 |doi: 10.2306/scienceasia1513-1874.2020.038

Structure and toxicity analysis of aflatoxin B1 biodegraded products by culture supernatant of Cladosporium uredinicola

Tian Ernuoa, Du Xina, Cai Wenhaob, Wang Changgaoa, Lin Jianguoa, Jun Caia,*

ABSTRACT:     Aflatoxin B1 (AFB1) is one of mycotoxins leading to serious food contaminations and human diseases. To solve these problems, biodegrading technique has been concerned. Previous researches showed that AFB1) can be effectively degraded by the culture supernatant of many microorganisms. However, the biodegraded products have not been identified. In this study, to explore the structure of the products, the AFB1) was degraded by the culture supernatant of Cladosporium uredinicola. Two biodegraded products of AFB1) were collected by high performance liquid chromatography (HPLC) and analyzed by liquid chromatography quadruple time-of-flight mass spectrometry (LC-Q-TOF MS) and chromatography-tandem mass spectrometry (MS/MS). As a result, the molecular weight of the two products were 406 and 342, and the structure of the two products were inferred. The toxicity of the biodegraded products was evaluated based on quantitative structure-activity relationship (QSAR) and cytotoxicity experiment. In conclusion, the double bonds of AFB1) had a high probability of being destroyed. The toxicity of the two biodegraded products compared with that of AFB1) was reduced. The culture supernatant of C. uredinicola was used as an effective way to detoxify AFB1).

Download PDF

57 Downloads 836 Views

a Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068 China
b College of Chemistry, Beijing Normal University, Beijing 100875 China

* Corresponding author, E-mail: hgdcaijun@ hbut.edu.cn

Received 6 May 2018, Accepted 25 Oct 2019