| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 49 Number 1
Volume 48 Number 6
Volume 48 Number 5
Volume 48 Number 4
Volume 48 Number 3
Volume 48 Number 2
Earlier issues
Volume 43 Number 3 Volume 43 Number 4 Volume 43 Number 5

previous article next article

Research articles

ScienceAsia 43 (2017): 240-243 |doi: 10.2306/scienceasia1513-1874.2017.43.240

Rapid detection of ancient human mitochondrial DNA variation using tetra-primer PCR amplification

Yongbin Zhaoa,*, Changchun Yua, Hongjie Lib, Ye Zhangb, Hui Zhoub

ABSTRACT:     Several methods have been used to diagnose the haplogroups in the maternal lineages of human remains. However, most techniques cannot be used to detect both the SNPs and the contamination. In this study, the tetra-primer PCR amplification method is used to type a number of coding-region mtDNA polymorphisms: G (4833), M9 (3394), and M10 (10 646). Results show that the tetra-primer PCR can rapidly identify mitochondrial DNA haplogroups in ancient human remains, and the result of amplification can be detected just by using agarose gel electrophoresis. Furthermore, this method can detect the existence of contamination under certain conditions.

Download PDF

89 Downloads 1517 Views

a Jilin Provincial Key Laboratory of Plant Resources Science and Green Production, Jilin Normal University, Siping, China
b College of Life Science, Jilin University, Changchun, China

* Corresponding author, E-mail: syding@jlnu.edu.cn

Received 12 Jun 2017, Accepted 13 Sep 2017