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Research articles

ScienceAsia 42(2016): 375-381 |doi: 10.2306/scienceasia1513-1874.2016.42.375

Cytotoxicity and genotoxicity of Allamanda and Plumeria species

Arunrat�Chaveeracha,b, Tawatchai�Taneeb,c, Natcha�Patarapadungkitb,d, Peerapat�Khamwachirapithakb, Runglawan�Sudmoonb,e,*

ABSTRACT:     Species of Allamanda and Plumeria have been used as medicinal plants for a long time. This study investigates the cytotoxicity and genotoxicity of their extracts on lymphocytes and HeLa cells using cell viability tests and comet assay. A.�cathartica, A.�violacea, P.�obtusa, and P.�rubra were collected and hexane-extracted. The maximum concentrations of crude extracts were 315, 625, 357, and 303�mg/ml, respectively. For cytotoxicity on lymphocytes treated with the maximum final concentration of the four extracts, the cell viabilities were 79%, 96%, 70%, and 60%, respectively. In HeLa cells, the IC50 was 13.5, 3.75, 21.5, and 14.5�mg/ml, respectively. These extracts showed significant genotoxicity (p<0.01) on both lymphocytes and HeLa cells. These findings suggest that Allamanda and Plumeria should not be used as medicinal plants.

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a Department�of�Biology, Faculty�of�Science, Khon�Kaen�University, Khon�Kaen�40002�Thailand
b Genetics�and�Environmental�Toxicology�Research�Group, Khon�Kaen�University, Khon�Kaen�40002�Thailand
c Faculty�of�Environment�and�Resource�Studies, Mahasarakham�University, Maha�Sarakham�44150�Thailand
d Department�of�Pathology, Faculty�of�Medicine, Khon�Kaen�University, Khon�Kaen�40002�Thailand
e Faculty�of�Law, Khon�Kaen�University, Khon�Kaen�40002�Thailand

* Corresponding author, E-mail: rsudmoon@yahoo.com

Received 27 May 2017, Accepted 0 0000