| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 43 Number 6
Volume 43 Number 5
Volume 43 Number 4
Volume 43 Number 3
Volume 43 Number 2
Volume 43 Number 1
Earlier issues
Volume 40 Number 5 Volume 40 Number 6 Volume 41 Number 1

previous article next article 1

Research articles

ScienceAsia 40(2014): 400-404 |doi: 10.2306/scienceasia1513-1874.2014.40.400

Distinctive roles of human Argonaute1, 2, 3, and 4 proteins on LINE-1 methylation and regulation of intragenic LINE-1-containing genes

Piyapat˙Pin-ona, Apiwat˙Mutirangurab,*

ABSTRACT:     Argonaute proteins (AGOs) are evolutionarily conserved and ubiquitously expressed in all higher eukaryotes. They participate in cell differentiation, transposon silencing, and other important functions. AGOs play key roles in the gene-silencing pathways guided by small RNAs. There are many human AGOs. However, redundancy and the distinctive roles of different AGOs have not been well characterized. Previously, we demonstrated that AGO2 down-regulates the expression of genes containing hypomethylated long interspersed nuclear elements (LINE-1s). Here, we evaluate the expression of genes containing LINE-1 in AGO1-4-knocked down HEK293 cells. Furthermore, we measured the methylation levels of AGO1-4-bound LINE-1s. Genes containing LINE-1s in AGO1, -2, -3 or -4-knocked down HEK293 cells showed prevented up-regulation, increased up-regulation, unchanged regulation and prevented up-regulation, respectively. Interestingly, AGO1-4 bound to LINE-1 differently in terms of the methylation level. Although the methylation level of AGO1 and -4-bound LINE-1s was not different from that of the genome, AGO2- and AGO3-bound LINE-1s were hypomethylated. Our experiments demonstrate the distinctive epigenetic roles of AGO1-4 in regulating genes containing LINE-1s.

Download PDF

0 Downloads 155 Views

a Inter-department˙Program˙of˙Biomedical˙Sciences, Faculty˙of˙Graduate˙School, Chulalongkorn˙University, Bangkok˙10330˙Thailand
b Centre˙of˙Excellence˙in˙Molecular˙Genetics˙of˙Cancer˙and˙Human˙Diseases, Department˙of˙Anatomy, Faculty˙of˙Medicine, Chulalongkorn˙University, Bangkok˙10330˙Thailand

* Corresponding author, E-mail: mapiwat@chula.ac.th

Received 22 Aug 2014, Accepted 2 Dec 2014