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Research articles

ScienceAsia 41(2015): 35-41 |doi: 10.2306/scienceasia1513-1874.2015.41.035


Cadmium and lead in seafood samples determined by solid phase extraction and graphite furnace atomic absorption spectrometry


Pipat Chootoa, Chalermpol Innuphata, Puchong Wararattananuraka,*, Chaipat Lapineeb

 
ABSTRACT:     A sample preparation method has been developed to detect cadmium and lead in commercial frozen seafood samples. After being digested with concentrated HNO3 in a closed polypropylene vessel, the clear sample solution was separated and preconcentrated by solid phase extraction using octadecyl silica membrane discs modified with 8-hydroxyquinoline prior to determination by graphite furnace atomic absorption spectrometry (GFAAS). The preconcentration conditions including pH, ligand quantity, eluent concentration, and volume were found to be effective with 10.0 mg of 8-hydroxyquinoline in 2.0 ml ethanol and pH 6.0. The retained cadmium and lead were efficiently eluted with 5.0 ml of 1.0 M HNO3 and determined by GFAAS. The detection limits for cadmium and lead were 0.073 µg/l and 0.332 µg/l, respectively. The proposed method was tested with the certified reference material, DORM-2 (Dogfish muscle), with good agreement. The recoveries were found to be 100–103% (n=4) for Cd and 92–108% (n=5) for Pb with relative standard deviation (n=5) of 3% for Cd and 4% for Pb. The extraction was found to be unaffected by coexisting ions. The method was applied satisfactorily to the seafood samples and trace levels of cadmium and lead were found.

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a Department of Chemistry, Analytical Chemistry Division, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla 90112 Thailand
b Department of Chemistry, School of Science, University of Phayao, Muang Phayao, Phayao 56000 Thailand

* Corresponding author, E-mail: puchong.w@psu.ac.th

Received 10 Aug 2014, Accepted 6 Feb 2015