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Volume 40 Number 5 Volume 40 Number 6 Volume 41 Number 1

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Research articles

ScienceAsia 40(2014): 414-419 |doi: 10.2306/scienceasia1513-1874.2014.40.414


Determination of gallic acid and rutin in extracts Cassia˙alata and Andrographis˙paniculata


Buran˙Phansawana,*, Supakorn˙Pongsabangphob

 
ABSTRACT:     This study determines gallic acid and rutin in ethanol, methanol, and acetic acid extracts of Cassia alata and Andrographis paniculata. A preliminary study using thin layer chromatography and 0.2% DPPH (2,2-diphenyl-1-picrylhydrazyl) spraying found that gallic acid was present in all three solvent extracts. Antioxidant components of all extractions were then analysed using high performance liquid chromatography with a C18 reverse-phase column at a flow rate of 0.50˙ml/min and 25˙°C. The results showed high components of gallic acid and rutin in all extracts of C.˙alata. The amounts of gallic acid found in C.˙alata extracted with ethanol, methanol, and acetic acid were 366, 410, and 258˙mg/g crude extract, respectively. Amounts of gallic acid found in A.˙paniculata extracted with ethanol, methanol, and acetic acid were 309.4, 351.2, and 309.1˙mg/g crude extract, respectively. Rutin concentrations found in C.˙alata extracted with ethanol, methanol and acetic acid were 194.4, 193.6, and 120.0˙mg/g crude extract, respectively. The highest gallic acid level was found in C.˙alata extracted with methanol, while the highest rutin levels were found in C.˙alata extracted with ethanol and methanol.

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a School˙of˙Energy˙and˙Environment, University˙of˙Phayao, Phayao˙56000˙Thailand
b Faculty˙of˙Medical˙Science, University˙of˙Phayao, Phayao˙56000˙Thailand

* Corresponding author, E-mail: buranphan@hotmail.com

Received 28 Jul 2014, Accepted 4 Dec 2014