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Research articles

ScienceAsia 41(2015): 28-34 |doi: 10.2306/scienceasia1513-1874.2015.41.028

Efficacy of Citrus˙hystrix sprays in decontaminating Streptococcus˙mutans on childrens toothbrushes

Kemthong˙Mitrakula,*, Ratchapin˙L.˙Srisatjalukb, Vimol˙Srisukhc, Kadkao˙Vongsawana

ABSTRACT:     Toothbrushes are sometimes contaminated by micro-organisms that can cause infection. This study evaluates the efficacy of Citrus hystrix sprays compared with 0.12% chlorhexidine gluconate (CHX) for disinfecting bacteria on children's toothbrushes. The participants were 61 children with a high caries risk aged 8–11. Each subject received two toothbrushes to be used continually for 7˙consecutive days. The first set of toothbrushes was used as a baseline without any disinfectant. The others were divided into 5˙groups and sprayed with distilled water (control), 0.12%˙CHX, 6%˙makrut oil (MO), 10%˙MO, or 13%˙MO. Toothbrushes were placed vertically into sterile test tubes containing 8˙ml of TYS20B medium and incubated overnight. Then we performed 10-fold serial dilutions from 10−1–10−5, and spread them on BHI or MS agars for investigating facultative bacteria, oral streptococci, and Streptococcus mutans. Results showed that for total facultative bacteria, 10%˙MO and 13%˙MO gave the highest reduction rate (100%)>CHX (88%)>6%˙MO (81%). For S.˙mutans, 10%˙MO and 13%˙MO also showed the highest reduction rate (100%)>6%˙MO (90%)>CHX (88%). For oral streptococci, 13%˙MO gave the highest reduction rate (100%)>10%˙MO (91%)>CHX (88%)>6%˙MO (69%). There was no significant difference between oral sprays and 0.12%˙CHX in the reduction of total facultative bacteria, oral streptococci, and S.˙mutans (p>0.05).

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a Department˙of˙Paediatric˙Dentistry, Faculty˙of˙Dentistry, Mahidol˙University, Bangkok˙10400˙Thailand
b Department˙of˙Oral˙Microbiology, Faculty˙of˙Dentistry, Mahidol˙University, Bangkok˙10400˙Thailand
c Department˙of˙Food˙Chemistry, Faculty˙of˙Pharmacy, Mahidol˙University, Bangkok˙10400˙Thailand

* Corresponding author, E-mail: mkemthong@yahoo.com

Received 17 Apr 2014, Accepted 6 Feb 2015