| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 44 Number 1
Volume 44 Number 1
Volume 43 Number 6
Volume 43 Number 5
Volume 43 Number 4
Volume 43 Number 3
Earlier issues
Volume 40 Number 6 Volume 41 Number 1 Volume 41 Number 2

previous article next article 1

Research articles

ScienceAsia 41(2015): 28-34 |doi: 10.2306/scienceasia1513-1874.2015.41.028


Efficacy of Citrus hystrix sprays in decontaminating Streptococcus mutans on childrens toothbrushes


Kemthong Mitrakula,*, Ratchapin L. Srisatjalukb, Vimol Srisukhc, Kadkao Vongsawana

 
ABSTRACT:     Toothbrushes are sometimes contaminated by micro-organisms that can cause infection. This study evaluates the efficacy of Citrus hystrix sprays compared with 0.12% chlorhexidine gluconate (CHX) for disinfecting bacteria on children's toothbrushes. The participants were 61 children with a high caries risk aged 811. Each subject received two toothbrushes to be used continually for 7 consecutive days. The first set of toothbrushes was used as a baseline without any disinfectant. The others were divided into 5 groups and sprayed with distilled water (control), 0.12% CHX, 6% makrut oil (MO), 10% MO, or 13% MO. Toothbrushes were placed vertically into sterile test tubes containing 8 ml of TYS20B medium and incubated overnight. Then we performed 10-fold serial dilutions from 10−110−5, and spread them on BHI or MS agars for investigating facultative bacteria, oral streptococci, and Streptococcus mutans. Results showed that for total facultative bacteria, 10% MO and 13% MO gave the highest reduction rate (100%)>CHX (88%)>6% MO (81%). For S. mutans, 10% MO and 13% MO also showed the highest reduction rate (100%)>6% MO (90%)>CHX (88%). For oral streptococci, 13% MO gave the highest reduction rate (100%)>10% MO (91%)>CHX (88%)>6% MO (69%). There was no significant difference between oral sprays and 0.12% CHX in the reduction of total facultative bacteria, oral streptococci, and S. mutans (p>0.05).

Download PDF

2 Downloads 235 Views


a Department of Paediatric Dentistry, Faculty of Dentistry, Mahidol University, Bangkok 10400 Thailand
b Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok 10400 Thailand
c Department of Food Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400 Thailand

* Corresponding author, E-mail: mkemthong@yahoo.com

Received 17 Apr 2014, Accepted 6 Feb 2015