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Research articles

ScienceAsia 41(2015): 5-11 |doi: 10.2306/scienceasia1513-1874.2015.41.005

Anti-inflammatory effect of Morinda citrifolia leaf extract on macrophage RAW 264.7 cells

Aurasorn Saraphanchotiwitthayaa,b,*, Pattana Sripalakitb,c

ABSTRACT:     Leaves of Morinda citrifolia (Rubiaceae) have been used in folk medicine to treat pain and inflammation. In this study, the anti-inflammatory activity of M. citrifolia leaf extracts was investigated. The extract was prepared by freeze-drying juice squeezed from M. citrifolia leaves. HPLC analysis of the crude extract revealed rutin to be a major compound together with small amounts of quercetin and kaempferol. The effect of this extract on TNF-α, IL-1β, and NO secretion by lipopolysaccharide-induced macrophage RAW 264.7 cells was investigated and compared to that of rutin, dexamethasone, and indomethacin. The results show that the extract inhibited TNF-α (98%, 750 µg/ml) secretion four times more than dexamethasone (3.92 µg/ml) and indomethacin (3.58 µg/ml), while rutin had no effect. The extract suppressed IL-1β secretion in a dose-dependent fashion with a maximum inhibition of about 79% (750 µg/ml). This was similar to that achieved by dexamethasone (97%, 3.92 µg/ml), indomethacin (70%, 3.58 µg/ml), and rutin (90%, 48.84 µg/ml). Moreover, the extract inhibited NO secretion (74%, 750 µg/ml) 50% more than rutin (40 µM) and 70% more than dexamethasone and indomethacin (10 µM). This study demonstrates the potential of M. citrifolia leaves as an effective therapeutic intervention against inflammatory diseases.

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a Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000 Thailand
b Pharmaceutical Biotechnology Research Unit, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000 Thailand
c Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000 Thailand

* Corresponding author, E-mail: aurasorns@nu.ac.th

Received 31 Mar 2014, Accepted 2 Dec 2014