Research articles
ScienceAsia 51 (2025):ID 2025054 1-9 |doi:
10.2306/scienceasia1513-1874.2025.054
Differential effects of chronic intermittent hypobaric hypoxia
on healthy and metabolic syndrome mice through liver tissue
proteomic analysis
Cheng Wanga, Wei Zhanga, Donghai Zhaoa, Pengyan Jia, Ying Changb, Dongfang Zhanga, Xinyu Jianga,
Mengxin Yua, Chunlian Dub, Yuling Qiub, Yingxin Zhangb, Lianhai Jina,*, Chunhong Suib,*
ABSTRACT: Thisstudyaimedtoexplore the differences in the effects of chronic intermittent hypobaric hypoxia (CIHH)
on healthy and metabolic syndrome (MetS) mice through proteomic analysis of liver tissue. Male C57BL/6J mice
were divided into four groups: CON (healthy mice), CIHH (healthy mice exposed to CIHH), MetS (MetS mice), and
MetS+CIHH (MetS mice exposed to CIHH). Mice in the MetS and MetS+CIHH groups were used to develop a MetS
model induced by a 16-week high-fat diet and 10% fructose water feeding. Then, mice in the CIHH and MetS+CIHH
groups were treated with CIHH (simulating 5000-m altitude for 28 days, 6 h daily). MetS diagnostic criteria were
routinely measured. Protein expression related to the hypoxia-inducible factor (HIF)-1 signaling pathway was assayed
byWesternblotting. Quantitativeproteomicanalysiswasperformedbymassspectrometry. CIHHsignificantlyimproved
body weight, abdominal circumference, Lee?s index, systolic blood pressure, fasting blood glucose, insulin resistance,
triglycerides, total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in MetS
mice, while these indicators had no statistical difference in healthy mice before and after CIHH treatment. HIF-1?
expression in the liver tissue was up-regulated only in MetS mice treated with CIHH. Proteomic analysis revealed
disparities in the characteristics of differentially expressed proteins between healthy and MetS mice under CIHH. In
summary, CIHH exhibited a significant capacity to improve MetS symptoms by up-regulating HIF-1? expression and
activating multiple metabolic and signaling pathways in MetS mice. However, the effects of CIHH on healthy mice
remained minimal.
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a |
School of Basic Medical Sciences, Jilin Medical University, Jilin 132013 China |
b |
School of Pharmacy, Jilin Medical University, Jilin 132013 China |
* Corresponding author, E-mail: lh_jin72@163.com, suichunhong@163.com
Received 29 Aug 2024, Accepted 0 0000
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