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Research articles

ScienceAsia 40(2014): 327-334 |doi: 10.2306/scienceasia1513-1874.2014.40.327


Immobilization of a thermophilic solvent-stable lipase from Acinetobacter baylyi and its potential for use in biodiesel production


Pakorn Winayanuwattikuna, Kingkaew Piriyakananona, Phenwarat Wongsathonkittikunb, Jittima Charoenpanichb,c,d,*

 
ABSTRACT:     Lipase transesterification of triglycerides is an environmentally safe alternative to chemical processing during biodiesel production. However, the cost and low stability of this enzyme remains problematic for commercial production. In this study, a thermophilic-solvent stable lipase from Acinetobacter baylyi (ABL) immobilized on Sepabeads EC-OD showed improved solvent stability. The optimal reaction conditions of immobilized ABL were comparable with those of the suspended lipase. Immobilization of ABL resulted in a broader pH activity range and enhanced storage stability. Optimal conditions for transesterification of palm oil were 6-step methanol feeding, 1:4 oil/methanol molar ratios, 20% enzyme loading, and 4% water content for 24 h at 40 °C. Conversions of oil feedstocks to biodiesel of between 13 and 93% were obtained. Reusability for transesterification of immobilized ABL was comparable to that of commercial lipases. This study found that immobilized ABL is one of the biocatalyst candidates for further development and application in enzyme-catalysed biodiesel synthesis.

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a Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330 Thailand
b Department of Biochemistry and Centre of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Burapha University, Bangsaen, Chonburi 20131 Thailand
c Environmental Science Program, Faculty of Science, Burapha University, Bangsaen, Chonburi 20131 Thailand
d Centre of Excellence on Environmental Health and Toxicology (CHE), Ministry of Education, Thailand

* Corresponding author, E-mail: jittima@buu.ac.th

Received 3 Jan 2014, Accepted 16 Sep 2014