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Research articles

ScienceAsia 40(2014): 379-383 |doi: 10.2306/scienceasia1513-1874.2014.40.379


Protocorm-like body proliferation of Grammatophyllum speciosum through asymbiotic seed germination


Sainiya Samalaa,*, Sompong Te-chatob, Sureerat Yenchonb, Kanchit Thammasiric

 
ABSTRACT:     Viability of Grammatophyllum speciosum seeds was evaluated by a TTC (2,3,5-triphenyl tetrazolium chloride) test. The seeds were then asymbiotically germinated on different agar media under aseptic conditions. The results showed that keeping the seeds in the dark at 30 °C for 1 day gave 99% of red staining embryos (referred to as viable). After sowing the seeds on half-strength Gamborg's B5 medium supplemented with 0.1% activated charcoal (AC) for 30 days, germination of those seeds was observed. Half-strength MS medium (1/2 MS) supplemented with 1 mg/l 6-benzylaminopurine in combination with 0.5 mg/l α-naphthalene acetic acid, 15% coconut water, 0.2% AC, and 0.7% agar gave the highest percentage of protocorm-like body (PLB) induction and proliferation. The PLBs could be regenerated into complete plantlets with vigorous shoot and root formation on 1/2 MS medium without any plant growth regulators. The survival rate of plantlets was 70% after hardening off in the greenhouse for 1 month.

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a Biology Program, Faculty of Science and Technology, Suratthani Rajabhat University, Suratthani 84100 Thailand
b Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai, Songkhla 90112 Thailand
c Department of Plant Science, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400 Thailand

* Corresponding author, E-mail: saineeya@hotmail.com

Received 8 Dec 2013, Accepted 16 Nov 2014