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Volume 40S Number 1 Volume 40 Number 1 Volume 40 Number 2

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Research articles

ScienceAsia 40 (2014): 35-41 |doi: 10.2306/scienceasia1513-1874.2014.40.035

Reduced albumin in the renal cortex of ethylene glycol-treated rats

Jui-Lung Shena,b,c, Kee-Ming Mana,d,e, Yung-Hsiang Chenf,g, Chiao-Hui Changf,g, Yuan-Ju Leeh, Huey-Yi Chenf,g, Kao-Sung Tsaif, Fuu-Jen Tsaif,g, Wen-Chi Chenf,g,*, Hsuan-Fu Kuoi

ABSTRACT:     Ethylene glycol (EG), a frequently used lithogenic agent, causes calcium oxalate (CaOx) crystal deposition in the renal cortex. In this study, we compared the protein expression pattern in kidneys of rat with EG-induced renal lithiasis with that of normal rats. Ten male Sprague-Dawley rats were divided into 2 groups. In the control group (n=5), rats were fed with normal drinking water; in the EG group (n=5), rats were fed with 0.75% EG in drinking water. After 4 weeks, rats fed with EG showed CaOx deposition in the renal cortex. The renal cortex proteins were extracted and separated by two-dimensional electrophoresis (2-DE). Comparative analyses of the respective spot patterns from the 2-DE output were performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Among the proteins identified, reduced albumin in rat renal lithiasis was confirmed by immunohistochemical analysis. Our results demonstrated that the amount of albumin was reduced in EG-treated rat kidneys. Subsequent crystal formation may be due to the loss of inhibitors or decreasing the remaining amount of albumin in the tissues, suggesting that albumin may play an important role in modulating EG-induced stone formation.

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a Department of Anesthesiology, Tung's Taichung Harbour Hospital, Taichung, Taiwan
b Centre for General Education, Feng Chia University, Taichung, Taiwan
c Department of Dermatology, Taichung Veteran General Hospital, Taichung, Taiwan
d Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan
e Graduate Institute of Geriatric Medicine, Anhui Medical University, Hefei, China
f Graduate Institute of Integrative Medicine, School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan
g Departments of Medical Research, Obstetrics and Gynecology, Pediatrics, and Urology, China Medical University Hospital, Taichung, Taiwan
h Department of Urology, National Taiwan University Hospital, Taipei, Taiwan
i Department of Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan

* Corresponding author, E-mail: wgchen@mail.cmu.edu.tw

Received 1 Apr 2013, Accepted 19 Oct 2013