ScienceAsia 40 (2014): 204-211 |doi:
Identification of phosphate-solubilizing bacteria from the bamboo rhizosphere
ABSTRACT: Some bacteria in the root zone of plants can solubilize phosphate making it available to the plant. Bacteria associated with bamboo roots may be a good source for development of bio-fertilizer for organic crop production. Here, 24 soil samples were collected from 8 species of bamboo, and 19 isolates of bacteria with the ability to solubilize phosphate were primarily selected based on the halo-to-colony ratio in Pikovskaya's medium. Five isolates with the highest ratios were cultured in vitro in a liquid medium containing mono-calcium phosphate (Ca(H2PO4)2) and soluble phosphate was then determined. The five isolates were identified using the full 16S rDNA sequencing method. Buttiauxella izardii had the highest soluble phosphate (134 mg/l) followed by Enterobacter cancerogenus (110 mg/l), Burkholderia ubonensis (106 mg/l), and E. hormaechei (103 mg/l), whereas Burkholderia pyrrocinia was lowest (38 mg/l). The isolates were further inoculated to soil planted with baby corn (Zea mays) in a pot experiment under an open environment. The experiment was not able to confirm the ability of these bacteria isolates to promote growth of the crop. The potential use of the isolates and the methods to improve the experiments for further studies are discussed.
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|Faculty of Science and Technology, Kanchanaburi Rajabhat University, Kanchanaburi 71190 Thailand
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Received 18 Mar 2013, Accepted 6 May 2014