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Research articles

ScienceAsia 39(2013): 631-635 |doi: 10.2306/scienceasia1513-1874.2013.39.631


Economical method for midiprep plasmid DNA purification using diatomaceous earth


Decha Sermwittayawong*, Chanawee Jakkawanpitak, Nureeya Waji, Nongporn Hutadilok-Towatana

 
ABSTRACT:     Diatomaceous earth has been used for DNA purification as it can produce high quality plasmid DNA. We describe an alternative and economical method for plasmid DNA purification from a 50-ml bacterial culture using diatomaceous earth, plastic pipette tips, conical tubes, centrifuges, and without requiring a vacuum system. Depending on the plasmid size and the origin of replication, this method yielded 200–800 µg plasmids with an A260/A280 of 1.8–2.0. These purified plasmids are suitable for many applications such as DNA sequencing and transfection experiments.

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Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai 90110 Thailand

* Corresponding author, E-mail: decha.s@psu.ac.th

Received 27 Feb 2013, Accepted 2 Sep 2013