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Research articles

ScienceAsia 39(2013): 574-583 |doi: 10.2306/scienceasia1513-1874.2013.39.574


Phylogenetic diversity of indigenous soya bean bradyrhizobia from different agro-climatic regions in Myanmar


Khin Myat Soea,*, Takeo Yamakawab, Shogo Hashimotoa, Papa Saliou Sarrc

 
ABSTRACT:     Soya bean (Glycine max Merr.) is the most important legume crop in the world and an important cash crop in Myanmar. In the present study, we characterized 43 isolates of indigenous soya bean rhizobia from Myanmar based on the sequence analysis of the bacterial 16S-23S rRNA internal transcribed spacer region. The sequence analysis confirmed that all isolates were categorized and identified as the genus Bradyrhizobium and they were conspecific with B. japonicum, B. elkanii, B. yuanmingense, and Bradyrhizobium sp. The highest genetic diversity of Bradyrhizobium strains was observed in the Shan State soya bean growing area. B. elkanii was distributed throughout the cultivation areas and was recorded as the dominant soya bean-nodulating strain in Myanmar. The present study revealed that there were two Rj-genotypes as Rj4- and non-Rj-genotypes among the collected soya bean cultivars from Myanmar. These findings indicate a high compatibility between Rj4-genotypes soya beans and nodulation type B strains in Shan State and Yangon region. In Mandalay region, the compatibility between non-Rj-genotype soya beans and nodulation type A strains was clearly investigated. This is the first report describing B. yuanmingense from nodules of soya bean and the geographical distribution of indigenous bradyrhizobia of soya bean from different regions in Myanmar.

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a Plant Nutrition Laboratory, Graduate School of Bioresources and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
b Plant Nutrition Laboratory, Division of Molecular Biosciences, Department of Biosciences & Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
c Centre for African Area Studies, Kyoto University, 46 Shimoadachi-cho, Sakyo-ku, 606-8501 Yoshida, Kyoto, Japan

* Corresponding author, E-mail: khinmyatsoe@gmail.com

Received 7 Feb 2013, Accepted 12 Jul 2013