ScienceAsia 40(2014): 193-197 |doi:
Antioxidant activity of flavonoids from leaves of Jatropha curcas
Qiulan Huang, Yiran Guo, Rao Fu, Tong Peng, Yuting Zhang, Fang Chen*
ABSTRACT: The objective of this study was to characterize the antioxidant properties of ethanol extracted from leaves of Jatropha curcas and to determinate the compounds. The IC50 values of DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt) were found to be 7.47 and 6.6 µg/ml, respectively, for the extracts. The reducing power of the extracts increased from 0.09 to 0.16, respectively, for 1–32 µg/ml of standard reagent. The assignment of the flavone compounds using HPLC-DAD-MSn was based on the evaluation of their UV-Vis absorption maxima (λmax) and mass spectral analyses. Tentatively, 4 compounds (orientin, vitexin, isovitexin, rhoifolin) were identified.
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|Key Laboratory Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, 610064 Chengdu, China
* Corresponding author, E-mail: Cfang@263.net
Received 25 Oct 2012, Accepted 22 Jan 2014