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Research articles

ScienceAsia 39(2013): 134-138 |doi: 10.2306/scienceasia1513-1874.2013.39.134


Efficient plant regeneration in vitro from cotyledon explants of chieh-qua (Benincasa hispida Cogn. var. chieh-qua)


E.E. Liua, David W.M. Leungb, Qing Hua Xiaa, Jie Ren Zhenga, Xin Xiang Penga, Xiao Ming Hec

 
ABSTRACT:     An efficient plant regeneration system for Benincasa hispida Cogn. var. chieh-qua was developed using explants isolated from mature seeds germinated on moist filter paper under aseptic conditions for 3 days in the dark and 2 days under light. Shoot regeneration frequency (percentage of explant-forming shoots) was about 90% when the proximal cotyledon portion with a 1 mm-long hypocotyl segment was first cultured on Murashige and Skoog (MS) medium supplemented with 6.0 mg/l benzylaminopurine (BA) and 0.2 mg/l naphthalene acetic acid (NAA) for 4 days and then transferred to MS medium containing 1 mg/l BA for 4 weeks. About 80% of the regenerated shoots could elongate further. The root induction medium was half-strength MS medium with 0.01 mg/l NAA and 0.01 mg/l BA. The average rooting frequency was about 93%.

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a College of Life Science, South China Agricultural University, Guangzhou 510642, China
b School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand
c Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

* Corresponding author, E-mail: xiaominghe@tom.com

Received 13 Aug 2012, Accepted 7 Jan 2013