Research articles
ScienceAsia 36 (2010): 286-291 |doi:
10.2306/scienceasia1513-1874.2010.36.286
Changes of anthocyanin cyanidin-3-glucoside content and antioxidant activity in Thai rice varieties under salinity stress
Wipavadee Daiponmaka,*, Piyada Theerakulpisutb, Pornthap Thanonkaoc, Apichart Vanavichitd, Preecha Prathephaa
ABSTRACT: This study was designed to determine the anthocyanin cyanidin-3-glucoside content and antioxidant activity of rice varieties under salinity stress. Two classes of Thai rice lines: cyanic (Riceberry, Kham, and Khamdoisaket) and acyanic (KDML 105, Sinlek, and BC2F7&hash;62-56) were hydroponically grown. Seedlings were grown for 16 days and were supplied with salinity nutrient solution with 0 (control) and 60 mM NaCl for 11 days. The total phenolic content and the antioxidant activity, including 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging, ferric reducing antioxidant power, and lipid peroxidation were also determined by colorimetric assay. Anthocyanin pigment cyanidin-3-glucoside was quantified by high performance liquid chromatography. The results show that salinity stress in Khamdoisaket and KDML 105 cultivars significantly (p<0.05) increased the total phenolic content, antioxidant activity, and cyanidin-3-glucoside content. Riceberry, Kham, and Sinlek cultivars, showed a moderate increase. The salt tolerant variety, BC2F7&hash;62-56, showed only a slight but not significant increase in antioxidant activity and virtually unchanged lipid peroxidation, total phenolic content, and anthocyanin cyanidin-3-glucoside content under salinity stress. These results suggest that the increased total phenolic content, antioxidant activity, and anthocyanin cyanidin-3-glucoside content in rice seedling might be a protection mechanism against salinity stress. BC2F7&hash;62-56 seems to have potential tolerance to salinity stress. This capability could be related to unchanged antioxidant activities, lipid peroxidation, total phenolic content, and anthocyanin cyanidin-3-glucoside content.
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| a |
Walai Rukhavej Botanical Research Institute, Mahasarakham University, Mahasarakham 44000, Thailand |
| b |
Department of Biology, Faculty of Science, Khonkaen University, Khonkaen 40002, Thailand |
| c |
Department of Biotechnology, Faculty of Technology, Khonkaen University, Khonkaen 40002, Thailand |
| d |
Rice Science Centre, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand |
* Corresponding author, E-mail: wipavadee.d@msu.ac.th
Received 18 Dec 2009, Accepted 23 Oct 2010
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