ScienceAsia 36 (2010): 194-198 |doi:
Detection of Tobacco streak virus by immunocapture-reverse transcriptase-polymerase chain reaction and molecular variability analysis of a part of RNA3 of sunflower, gherkin, and pumpkin from Andhra Pradesh, India
Bheemathati Sarovar*, Y. Siva Prasad, D.V.R. Sai Gopal
ABSTRACT: The coat protein-coding and 3′UTR regions of the RNA3 of Tobacco streak virus infecting sunflower, gherkin, and pumpkin with the characteristic symptoms of necrosis were amplified by IC-RT-PCR. The amplicons were cloned and sequenced. The nucleotide sequences of TSV clones were determined as ∼1 kb. This length corresponds to 717 and 288 nucleotides of coat protein-coding and 3′UTR regions, respectively. Comparative sequence analysis of the coat protein-coding region of TSV isolates under study both at the nucleotide and the amino acid levels indicated 98±1% and 97±1% identity, respectively, with TSV reported from other hosts. The sequence analysis of the 3′UTR region at the nucleotide level showed 98±1% and 88% identities with the Indian and US isolates, respectively. IC-RT-PCR was found to be more sensitive than RT-PCR, and hence could be used in quarantine programmes.
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|Department of Virology, S.V. University, Tirupati-517 502, Andhra Pradesh, India
* Corresponding author, E-mail: firstname.lastname@example.org
Received 14 Oct 2009, Accepted 10 Aug 2010