ScienceAsia 48 (2022): 558-567 |doi:
Chrysophanol induces cell apoptosis and suppresses cell
invasion by regulating AKT and MAPK signaling pathway in
Jian Guana, Hemin Huangb,*
ABSTRACT: Melanoma is a highly heterogeneous and invasive tumor with poor prognosis due to its resistance to
radiotherapy, chemotherapy, and immunotherapy. Chrysophanol has the anti-carcinogenic role in a variety of tumors.
Although the anti-proliferative role of chrysophanol in melanoma has been reported, its specific molecular mechanisms
are still unclear. In the present study, the proliferation, migration, invasion, apoptosis, and cell cycle of A375 and A2058
cells were evaluated through MTT, colony formation, cell scratch, Transwell, and flow cytometry assays. Besides,
the relative levels of proteins related to AKT and MAPK signaling pathway were determined by western blotting.
The results revealed that chrysophanol significantly inhibited cell viability, colony formation, and cell invasive but
increased the relative wound width of A375 and A2058 cells. Chrysophanol also notably enhanced the apoptotic
rate, accompanied with the induced protein levels of Bax and Cleaved caspase-3 and reduced protein level of Bcl-2. In
addition, chrysophanol observably promoted the ratio of G0/G1 phase, while restrained the relative protein expressions
of Cyclin D1 and CDK4. Furthermore, the relative expressions of p-AKT/AKT, p-ERK1/2/ERK1/2, and p-JNK/JNK were
significantly decreased, and the relative expression of p-38/38 was enhanced by 20, 50, and 100 ?M chrysophanol
treatment. Totally, the dose-dependent effects of chrysophanol on melanoma cell proliferation, migration, invasion,
apoptosis, and cell cycle were observed. To sum up, these results revealed that chrysophanol induced cell apoptosis
and suppressed cell invasion by regulating AKT and MAPK signaling pathway in both A375 and A2058 cells in a dosedependent manner.
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||Department of Cosmetic and Plastic Burn Wound Repair, Lishui People?s Hospital, Lishui, Zhejiang 323020 China
||Department of Burn Plastic Wound Repair, Chongqing Qianjiang Central Hospital, Chongqing 409000 China
* Corresponding author, E-mail: firstname.lastname@example.org
Received 20 Oct 2021, Accepted 27 Feb 2022