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Volume 47 Number 6 Volume 48 Number 1

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Research articles

ScienceAsia 48 (2022): 62-68 |doi: 10.2306/scienceasia1513-1874.2022.012

Comparative studies of anthocyanin accumulation and gene expression of flavonoid 30' -hydroxylase during fruit development of two Praratchatan strawberry cultivars

Mongkon Sirijan, Peerasak Chaiprasart*

ABSTRACT:     Anthocyanins in strawberry fruit are beneficial to human health. Flavonoid 3'-hydroxylase (F3'H) is a key enzyme in determining the B-ring hydroxylation pattern of flavonoids involved in the anthocyanin production pathway of strawberry fruit. The objectives of this study were to determine the correlation between the types and the amounts of anthocyanins and the expression levels of F3'H at four developmental stages of strawberry fruit in two different cultivars: Praratchatan No. 70 and Praratchatan No. 80. The concentration of cyanidin-3-glucoside (cy-3-glc) in the fruit extract samples was analysed using HPLC. Gene expression was determined using RT-qPCR. Colour and pH were measured at each developmental stage. The cy-3-glc concentration was significantly different at each developmental stage (p < 0.05), and higher in the Praratchatan No. 70 than in the Praratchatan No. 80. The concentration gradually increased from the early green stage (15 days after anthesis, DAA), to the maximum at the turning stage (25 DAA), and then decreased slightly at the ripe red stage (30 DAA). Gene expression of F3'H was positively correlated with the concentration of cy-3-glc at each stage of fruit development. The ripe red stage (25 DAA) was found to be the optimal time to harvest the fruit for healthy consumption.

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a Center of Excellence in Postharvest Technology, Commission of Higher Education, Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok 65000 Thailand

* Corresponding author, E-mail: peerasakc@gmail.com

Received 2 May 2020, Accepted 20 Aug 2021