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Research articles

ScienceAsia 46S (2020): 27-35 |doi: 10.2306/scienceasia1513-1874.2020.S004

Biological activity of the thrombospondin epidermal growth factor domain from banana shrimp (Fenneropenaeus merguiensis)

Monwadee Wonglapsuwana,b, Natthaporn Khwanmuanga, Panchalika Deachamaga,b, Wilaiwan Chotigeata,b,*

ABSTRACT:     Human epidermal growth factor (EGF) plays a role in cell proliferation and has been used to promote wound healing in patients. However, its role in cancer development via the EGF receptor (EGFR) has recently been reported. Thrombospondin (TSP) from banana shrimp (Fenneropenaeus merguiensis) contains an EGF domain at its carboxy-terminal end though it shares only 30% nucleotide sequence similarity with human EGF. It is challenging to investigate its biological activities. In this work, the EGF domain from banana shrimp TSP (TSP3-2) was cloned, and the recombinant His-TF-TSP3-2 was evaluated for its effects on proliferation, cytotoxicity and collagen production in a skin fibroblast cell line. Additionally, genotoxicity was determined using a hypoxanthine-guanine phosphoribosyltransferase mutation assay. Fibroblast cell proliferation increased 1.21.4 fold after treatment with 0.515 µg/mL His-TF-TSP3-2. The migration rate after 48 h of the His-TF-TSP3-2-treated cells (at 0.5 µg/mL) in a scratch assay was greater than that of the control. The collagen production was 1.7- and 1.2-fold increased after treatment with His-TF-TSP3-2 at 0.5 and 1.0 µg/mL, respectively. Low toxicity to the genome and no significant mutant frequency was found in the His-TF-TSP3-2-treated cells. Further investigations are required to confirm a potential use for this protein.

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a Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Songkhla 90112 Thailand
b Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Songkhla 90112 Thailand

* Corresponding author, E-mail: wilaiwan58@hotmail.com

Received 7 Sep 2019, Accepted 24 Jan 2020