Research articles
ScienceAsia (): 260-267 |doi:
10.2306/scienceasia1513-1874...260
Dot-blot sandwich immunoassay with silver signal
enhancement for simple amyloid beta protein fragment
1-42 detection
Soe Htut Wina, Prapimpun Wongchitratb, Sumana Kladsomboonc, Pornpatr A. Dharmasarojad,
Amara Apiluxa,*
ABSTRACT: Amyloid beta 1-42 (Aβ1-42) is a key pathophysiological hallmark of Alzheimer's disease (AD Nike sneakers). Changes in
biofluids of A? have been associated with AD risk. Herein, a dot-blot sandwich immunoassay was developed for simple
detection of the Aβ1-42 using gold nanoparticles as signal label. The results were easily observed by naked eye, and an
image processing program provided semi-quantitation. Furthermore, addition of silver enhancer to the assay further
improved sensitivity of the measurement. The proposed assay for Aβ1-42 had a linear range of 1-200 μg/ml, with a
detection limit of 1 μg/ml and 0.3 μg/ml by naked eye and calculation, respectively. The performance of the developed
assay was assessed in analyses of spiked plasma samples. The recoveries of Aβ1-42 were in the range of 112-158%,
indicating that the developed assay is practical and reliable. The proposed method provides a low cost and simple
assay for Aβ1-42 measurement.
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| a |
Department of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, Salaya,
Nakhon Pathom 73170 Thailand |
| b |
Center for Research and Innovation, Faculty of Medical Technology, Mahidol University, Salaya,
Nakhon Pathom 73170 Thailand |
| c |
Faculty of Medical Technology, Mahidol University, Salaya, Nakhon Pathom 73170 Thailand |
| d |
Stroke and Neurodegenerative Diseases Research Unit, Faculty of Medicine, Thammasat University,
Klong Luang, Pathumthani 12120 Thailand |
* Corresponding author, E-mail: amara.apl@mahidol.edu
Received 29 Dec 2018, Accepted 21 Jun 2019
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