ScienceAsia 31 (2005): 323-328 |doi: 10.2306/scienceasia1513-1874.2005.31.323
Phylogenetic Diversity of Wild Edible Russula from
Northeastern Thailand on the Basis of Internal
Transcribed Spacer Sequence
Monchai Manassila,a Thanwalee Sooksa-Nguan,a Nantakorn Boonkerd,a Sureelak Rodtongb and
ABSTRACT:Phylogenetic relationships among wild edible Russula in northeastern Thailand were demonstrated
on the basis of the sequence polymorphisms in the internal transcribed spacer (ITS) region of the nuclear
ribosomal genes (rDNA). Using PCR-RFLP analysis, they could be divided into three main clusters. ITS
sequences of some specimens were also analyzed. The ITS sequences clearly distinguished most specimens
from each other, even those sharing similar morphotypes. This approach might be used for further investigation
of ectomycorrhizal associations in the forest regeneration programme in Thailand.
a School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology,
Nakhon Ratchasima 30000, Thailand.
b School of Microbiology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima
# Both authors contributed equally to the work.
* Corresponding author, E-mail: email@example.com
Received 24 Dec 2004,
Accepted 10 Jun 2005