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Research Article

ScienceAsia 31 (2005): 323-328 |doi: 10.2306/scienceasia1513-1874.2005.31.323

Phylogenetic Diversity of Wild Edible Russula from Northeastern Thailand on the Basis of Internal Transcribed Spacer Sequence

Monchai Manassila,a Thanwalee Sooksa-Nguan,a Nantakorn Boonkerd,a Sureelak Rodtongb and Neung Teaumroonga*

ABSTRACT:Phylogenetic relationships among wild edible Russula in northeastern Thailand were demonstrated on the basis of the sequence polymorphisms in the internal transcribed spacer (ITS) region of the nuclear ribosomal genes (rDNA). Using PCR-RFLP analysis, they could be divided into three main clusters. ITS sequences of some specimens were also analyzed. The ITS sequences clearly distinguished most specimens from each other, even those sharing similar morphotypes. This approach might be used for further investigation of ectomycorrhizal associations in the forest regeneration programme in Thailand.

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a School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology,
Nakhon Ratchasima 30000, Thailand.
b School of Microbiology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima
30000, Thailand.

# Both authors contributed equally to the work.
* Corresponding author, E-mail: neung@sut.ac.th

Received 24 Dec 2004, Accepted 10 Jun 2005