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Research Article

ScienceAsia 29 (2003): 135-140 |doi: 10.2306/scienceasia1513-1874.2003.29.135

Applicaion of Factorial Design to Study the Effects of Temperature, Iitial pH and Agitation on the Production of Cyclodextrin Glucanotransferase from Alkalophilic Bacillus sp. G1

Rosli Md Illiasa,*, Tein Siew Fena, Roshanida A Rahmana, Noor Aini Abdul Rashidb, Wan Mohtar Wan Yusoffc, Aidil Abd Hamidc, Osman Hassand and Kamarukzaman Kamaruddine


ABSTRACT: Production of cyclodextrin glucanotransferase (CGTase) from Bacillus sp. G1 was investigated. Two level Full Factorial Design has been employed to study the effect of different experimental variables on the production of CGTase. Three variables of pH (8, 9, and 10), temperature (32 oC, 34.5 oC, and 37 oC) and agitation (100 rpm, 200 rpm, and 300 rpm) were used to identify the significant effects and interactions in the batch studies. A polynomial regression model has been developed using the experimental data. The results show that production of CGTase was strongly affected by the variations in pH. The maximum CGTase activity was achieved when the production was carried out at 32oC, pH 10 with 100 rpm of agitation. The predicted value produced 27.85 U/ml is in close agreement with CGTase activity produce from experiment, which is 27.82 U/ml.

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a Department of Bioprocess Engineering, Faculty of Chemical and Natural Resources Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.
b Department of Biology, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia.
c School of Bioscience and Biotechnology and
d School of Chemical Science and Food Science, Faculty Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
e Bioprocess and Chemical Technology Centre, SIRIM Berhad, 40911, Shah Alam, Malaysia.

* Corresponding author, Email: r-rosli@utm.my

Received 11 Apr 2002, Accepted 24 Jan 2003