Research articles
ScienceAsia 52 (2026): 1-8 |doi:
10.2306/scienceasia1513-1874.2026.049
Glyphosate detection by specific inhibition of the nanozyme
activity of MOF-808-NH2
: A dual-mode sensor with enhanced
reliability for analysis
Chuhan Bian
ABSTRACT: This study successfully constructed an efficient colorimetric/fluorometric dual-mode nanozyme sensor
based on a metal-organic framework (MOF-808-NH2
) for the highly selective and sensitive detection of glyphosate
(GP). The sensor leverages the dual functionalities of MOF-808-NH2
, namely its intrinsic peroxidase-like activity and
blue fluorescence emission. In the presence of hydrogen peroxide (H2O2
), MOF-808-NH2
catalyzes the oxidation
of colorless 3,3?
,5,5?
-tetramethylbenzidine (TMB) to generate blue oxTMB, producing a distinct colorimetric signal.
Simultaneously, the generated oxTMB quenches the inherent blue fluorescence of MOF-808-NH2
via the inner filter
effect (IFE). GP specifically inhibits the peroxidase-like activity of MOF-808-NH2
, leading to a reduction in oxTMB
generation. This inhibition results in a dual-mode response: a weakening of the blue color (decreased colorimetric
signal) and a recovery of the blue fluorescence (enhanced fluorescence signal). This dual-signal response strategy
effectively integrates the intuitiveness of colorimetric analysis with the high sensitivity of fluorescence detection,
eliminating the need for unstable biological enzymes, demonstrating good reliability, accuracy, and broad application
prospects in the analysis of real samples. It provides a core driving force for achieving comprehensive connectivity
between precision agriculture and environmental health.
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School of Chemical Engineering and Technology of Tiangong University, Tianjin 300387 China |
* Corresponding author, E-mail: 2411510215@tiangong.edu.cn
Received 24 Nov 2025, Accepted 30 Apr 2026
|
