Research articles
ScienceAsia 51 (2025):ID 2025072 1-8 |doi:
10.2306/scienceasia1513-1874.2025.072
Comparative analysis of proteomic changes during cadmium
stress metabolic responses by quantitative iTRAQ in maize
(Zea mays L.) seedling roots
Haoyu Youa,b,?, Xiang Chenc,?, Lin Yangd, ChangJun Guoe, Min Xuf,*, Hua Penga,*
ABSTRACT: Cadmium (Cd), a nonessential heavy metal, has become the most important metal pollutant in soils. Cd
inhibits plant growth and development and causes substantial reductions in crop yield. However, limited information
is available regarding the heavy metal stress-related proteome of maize. We aimed to analyze the proteomic alterations
induced by Cd exposure during maize root development to identify potential genes or pathways related to Cd stress.
Consequently, an isobaric tag for relative and absolute quantification (iTRAQ)-based proteomic approach was used to
analyze the protein expression from seedling roots of the non-hyperaccumulator inbred line B73 and hyperaccumulator
inbred line Mo17 at four developmental stages (0, 12, 24, and 48 h) under heavy metal cadmium pollution. The results
showed that 209, 294, 422, and 384, 352, 489 proteins were screened as differentially expressed proteins (DEPs)
between the control and Cd-treated roots for 12, 24, and 48 h in B73 and Mo17, respectively. Ten DEPs were identified
as commonly expressed in both B73 and Mo17, including Ribosomal protein L15, L17c, and L36, Peroxidase 3, GST,
ADP-ribosylation factor A1B, voltage-dependent anion channel protein 1b, among others. In B73, DEPs related to
ribosomal protein synthesis were significantly upregulated, while they were downregulated in Mo17. Simultaneously,
CRPs associated with antioxidant activity were identified, suggesting that B73 might enhance cadmium tolerance by
improving protein synthesis and ROS scavenging capacity. These discoveries will offer valuable perspectives into the
molecular mechanisms of maize responses to cadmium toxicity and detoxification at the protein level.
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| a |
Sichuan Tourism University, Chengdu, Sichuan 610100 China |
| b |
College of Resources, Sichuan Agricultural University, Wenjiang, Sichuan 611130 China |
| c |
School of Public Health, Southwest Medical University, Luzhou, Sichuan 646000 China |
| d |
Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066 China |
| e |
Agriculture and Rural Affairs Bureau of Dongxing District, Sichuan 610016 China |
| f |
Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College,
Chengdu 610052 China |
* Corresponding author, E-mail: ph82107@163.com
Received 20 Jan 2024, Accepted 0 0000
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