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Volume 44 Number 5
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Volume 39 Number 3 Volume 39 Number 4 Volume 39 Number 5

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ScienceAsia 39(2013): 444-447 |doi: 10.2306/scienceasia1513-1874.2013.39.444


Comparison of second-derivative spectrophotometry and HPLC for determination of amygdalin in wild apricot kernels


Xingjun Miao, Zhong Zhao*, Hailan Zhu, Ming Li, Qingxia Zhao

 
ABSTRACT:     Amygdalin is a cyanogenic glucoside found in apricot kernels. To facilitate the exploitation and use of wild apricot resources, it is necessary to be able to rapidly and efficiently determine the amygdalin levels within their kernels. To do so, this paper compares two methods, one based on second-derivative UV spectrophotometry and the other on high-performance reversed-phase liquid chromatography (HPLC). The second-derivative spectrophotometry method effectively eliminates background noise and is straightforward to perform while retaining the accuracy of the HPLC method. It is likely to be a useful tool for the large-scale determination of amygdalin concentrations in wild apricot kernels and press cake.

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Key Laboratory of Environment and Ecology in Western China of Ministry of Education, College of Forestry, Northwest A & F University, 712100, China

* Corresponding author, E-mail: zhaozh@nwsuaf.edu.cn

Received 8 Jan 2013, Accepted 10 Apr 2013