| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 45 Number 2
Volume 45 Number 1
Volume 44 Number 6
Volume 44 Number 5
Volume 44 Number 4
Volume 44 Number 3
Earlier issues
Volume 39 Number 3 Volume 39 Number 4 Volume 39 Number 5

previous article 1

Short reports

ScienceAsia 39(2013): 444-447 |doi: 10.2306/scienceasia1513-1874.2013.39.444

Comparison of second-derivative spectrophotometry and HPLC for determination of amygdalin in wild apricot kernels

Xingjun Miao, Zhong Zhao*, Hailan Zhu, Ming Li, Qingxia Zhao

ABSTRACT:     Amygdalin is a cyanogenic glucoside found in apricot kernels. To facilitate the exploitation and use of wild apricot resources, it is necessary to be able to rapidly and efficiently determine the amygdalin levels within their kernels. To do so, this paper compares two methods, one based on second-derivative UV spectrophotometry and the other on high-performance reversed-phase liquid chromatography (HPLC). The second-derivative spectrophotometry method effectively eliminates background noise and is straightforward to perform while retaining the accuracy of the HPLC method. It is likely to be a useful tool for the large-scale determination of amygdalin concentrations in wild apricot kernels and press cake.

Download PDF

3 Downloads 334 Views

Key Laboratory of Environment and Ecology in Western China of Ministry of Education, College of Forestry, Northwest A & F University, 712100, China

* Corresponding author, E-mail: zhaozh@nwsuaf.edu.cn

Received 8 Jan 2013, Accepted 10 Apr 2013